COI Illumina NextSeq Metabarcoding Library: Processed Reads for Ecological Analysis

Processed reads from a COI Illumina NextSeq metabarcoding library, including true samples, positive controls, and negative controls. The data was processed using the R package dada2 and formatted for ecological analyses with packages like phyloseq and vegan. Author Alejandro Maeda Obregon last updated the dataset on 2026-04-28.

Use Cases

• Taxonomic classification of species based on processed COI gene sequences.
• Ecological community analysis using the phyloseq-formatted data.
• Quality control assessment for metabarcoding workflows based on included positive and negative controls.
• Biodiversity pattern analysis leveraging the vegan-formatted ecological data.

Strengths

• Includes true samples alongside positive and negative controls, which can aid in quality assessment.
• Data is processed and formatted for ecological analysis using established R packages (dada2, phyloseq, vegan).
• Released under a permissive CC-BY-4.0 license.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Processed reads from an Illumina NextSeq run, analyzed with the R package dada2.

Freshness

Last updated 2026-04-28 12:44:02; freshness should be verified.