Fluorescence Polarization-Based High-Throughput Screening Enables Identification of Vibrio

Bo Jin's dataset from 2026 contains results from a fluorescence polarization-based high-throughput screening assay used to identify inhibitors of the Vibrio histidine kinase VbrK. The data, stored in a PDB file of 622.2 KB, underpins the discovery of LPZ-51, a compound shown to potentiate β-lactam antibiotics and improve host survival in a zebrafish infection model.

Use Cases

• Training models to predict kinase inhibitor activity based on fluorescence polarization assay results.
• Analyzing structure-activity relationships for VbrK inhibitors mentioned in the study.
• Benchmarking virtual screening methods against experimental high-throughput screening data.

Strengths

• Data is associated with a peer-reviewed study demonstrating in vitro and in vivo efficacy of the identified inhibitor LPZ-51.
• The dataset has a specific mechanistic focus on blocking β-lactamase induction at the transcriptional level.
• File format (PDB) suggests the data may include structural information relevant for molecular modeling.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale ML tasks.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small in scale at 622.2 KB, indicating limited scope.

Provenance

Source

figshare, author Bo Jin.

Collection Method

Generated via an optimized fluorescence polarization-based high-throughput screening assay followed by hit-to-lead optimization.

Time Range

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Freshness

Last updated 2026-04-30 07:04:08.

Geography

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