Fluorescence Polarization-Based High-Throughput Screening Enables Identification of Vibrio

A dataset from a high-throughput screening assay to identify inhibitors of the VbrK sensor kinase in Vibrio bacteria. The research demonstrates that the inhibitor LPZ-51 blocks β-lactamase induction at the transcriptional level and synergizes with carbenicillin in a zebrafish infection model. The dataset was authored by Bo Jin and last updated on April 30, 2026.

Use Cases

• Training models to predict compound activity from high-throughput screening data based on the fluorescence polarization assay described.
• Analyzing structure-activity relationships for VbrK kinase inhibitors based on the hit-to-lead optimization process mentioned.
• Evaluating synergy between novel inhibitors and β-lactam antibiotics based on the described potentiation of antibiotic activity.

Strengths

• Dataset is small (17.5 KB), facilitating quick download and initial exploration.
• Specific experimental results are described, including the identification of the effective inhibitor LPZ-51.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Data likely originates from a fluorescence polarization-based high-throughput screening assay followed by hit-to-lead optimization, as described.

Time Range

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Freshness

Last updated 2026-04-30 07:04:09; freshness should be verified.

Geography

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