Fluorescent Optrode Protein Detection Data from Urine Samples

A linear dynamic range from 10 mg L-1 to 100 mg L-1 for protein detection is reported. The dataset describes a fluorescent optrode sensor based on a diketopyrrolopyrrole derivative, tested for total protein determination in urine samples from healthy individuals. Results were validated against a Ponceau-S/TCA spectrophotometric method by Marta Veríssimo from the University of Aveiro.

Use Cases

• Benchmarking new protein detection methods based on the reported linear dynamic range and detection limit.
• Developing reagent-efficient clinical assays based on the described reversibility and lack of need for trichloroacetic acid treatment.
• Assessing sensor interference resistance based on the described non-interference from sodium dodecyl sulfate, acetone, glucose, and fluoride ion.

Strengths

• Detection limit is reported to be better than the classical Biuret method and similar to the Ponceau-S/TCA method.
• The method offers advantages in economy of reagents and time due to the reversibility of the recognition layer.
• The sensor's response is reportedly unaffected by common interferences like sodium dodecyl sulfate, acetone, glucose, and fluoride ion.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset's scale is unknown, which may limit suitability assessment for large-scale model training.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Aveiro

Collection Method

Experimental data from a designed fluorescent sensor tested on urine samples.