SaCas9 PAM Relaxation Design: From NNGRRT to NNNRRT

UniDesign program and result summary for the computational redesign of SaCas9 to relax its PAM requirement. The work was conducted by Xiaoqiang Huang at the University of Michigan. The repository includes scripts and a summary of the design outcomes.

Use Cases

• Training protein language models based on the designed SaCas9 variants.
• Benchmarking computational protein design algorithms using the provided result summary.
• Developing new genome editing tools with relaxed PAM requirements based on the described methodology.

Strengths

• Includes the UniDesign program, enabling reproduction and extension of the work.
• Result summary provides a concrete outcome of the PAM relaxation from NNGRRT to NNNRRT.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Michigan

Collection Method

Computational design using the UniDesign program.