Alfalfa Gene Expression and Phenotype Data Under Salt Stress

Yiwen Lu published a dataset on figshare in 2026 detailing a functional analysis of ARR, MYC, and FLS genes in alfalfa. The data includes results from experiments using Agrobacterium rhizogenes-mediated composite plants subjected to short-term salt stress (150 mmol/L NaCl for 1 hour). It contains measurements of transgenic root induction efficiency, stress response phenotypes, and total flavonoid content across different gene overexpression lines.

Use Cases

• Compare the stress response efficiency of different Agrobacterium rhizogenes strains based on the described root-tip coating method.
• Analyze the role of flavonoid-associated genes in early salt stress tolerance based on the described phenotypic and biochemical assays.
• Validate candidate genes for rapid functional screening based on the described composite plant system platform.
• Investigate the correlation between specific gene overexpression (ARR, MYC, FLS) and flavonoid accumulation under stress conditions.

Strengths

• Data is associated with a specific, controlled experimental protocol using 150 mmol/L NaCl for 1 hour.
• The dataset includes results from comparing two Agrobacterium rhizogenes strains (K599 and Arqua1) for transformation efficiency.
• Findings are linked to a previously published transcriptome atlas, providing a basis for candidate gene selection.

Limitations

• The dataset is small (2.4 MB), which may limit the scope of analysis.
• Row count and column-level documentation are unknown, requiring manual inspection after download.
• The description does not specify the number of biological or technical replicates used in the experiments.

Provenance

Source

figshare, author Yiwen Lu.

Collection Method

Experimental data from Agrobacterium rhizogenes-mediated transformation and salt stress assays on alfalfa composite plants.

Freshness

Last updated 2026-04-25 13:22:27.