Functional Analysis of ROX1 and rolB Genes in Tobacco Floral Morphology

A 1002 KB PDF document authored by Kenta Tsuchiyama, last updated on 2026-05-25, presents research on the functional analysis of the ROX1 and rolB genes in Nicotiana tabacum (tobacco). The study investigates the roles of these genes in floral organ elongation by analyzing plants with ROX1 overexpression or gene knockout, generated using CRISPR-Cas9 editing.

Use Cases

• Study gene expression effects on floral organ length based on ROX1 overexpression and knockout experiments.
• Investigate the interaction between the rolB oncogene and ROX1 orthologs in causing floral abnormalities.
• Analyze the role of CRISPR-Cas9 gene editing in functional plant genomics based on the described ROX1-KO model.

Strengths

• Dataset includes specific, quantified measurements of floral organ lengths (e.g., petal length reduced to 74%, filament to 67%).
• Research compares multiple genetic conditions: wild type, ROX1-OX, ROX1-KO, WT-RolB, and ROX1-KO-RolB.
• License is clearly defined as CC-BY-4.0, permitting open reuse with attribution.

Limitations

• Dataset is a single PDF document (1002 KB); underlying raw data and column-level documentation are absent.
• Row count and data structure are unknown, limiting suitability assessment for computational analysis.
• Freshness should be verified; last update was 2026-05-25 11:53:19.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from genetic manipulation and phenotypic measurement of tobacco plants.

Freshness

Last updated 2026-05-25 11:53:19.