Chlamydomonas Herbicide Tolerance Genes from Genome-Wide Screening

A genome-wide functional genomics screen in the green alga Chlamydomonas reinhardtii investigated tolerance to the herbicides paraquat and diuron. The dataset, authored by Tim Godec and last updated in May 2026, successfully uncovered nuclear-encoded pathways governing susceptibility, including genes for photosynthetic machinery assembly and novel non-target-site resistance mechanisms. The findings provide mechanistic insights to advance ecotoxicological risk assessments.

Use Cases

• Identify candidate genes for herbicide tolerance based on genome-wide screening results.
• Study nuclear control over chloroplast-localized targets related to photosynthetic machinery.
• Investigate intracellular trafficking pathways as determinants of paraquat toxicity.
• Analyze metabolic strategies for energy conservation linked to diuron tolerance.
• Validate novel non-target-site resistance mechanisms proposed by the functional genomics approach.

Strengths

• Dataset is the product of a genome-wide functional genomics screen, providing an unbiased approach.
• Specific molecular pathways are identified, including a P5B ATPase transporter and a fatty acid elongation pathway.
• The dataset is focused on two well-characterized herbicides, enabling comparative analysis of tolerance mechanisms.
• It is shared under a CC-BY-NC-4.0 license, allowing for clear reuse with attribution for non-commercial purposes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• The dataset is very small (14.9 KB), indicating limited scope or a summary-level output.

Provenance

Source

figshare

Collection Method

Genome-wide functional genomics screening in Chlamydomonas reinhardtii.

Freshness

Last updated 2026-05-27 06:43:49; freshness should be verified.