Gene Expression in Human Fibroblasts After DNA Damage in Space

NASA's dataset profiles gene expression changes in confluent human fibroblasts (AG01522) exposed to microgravity on the International Space Station and treated with the DNA-damaging agent bleomycin. Cells were treated for 3 hours with 1.0 µg/ml bleomycin after three days on the ISS, then fixed and returned to Earth for RNA isolation and microarray analysis. The dataset was last updated on March 13, 2026.

Use Cases

• Identify genes differentially expressed due to microgravity based on the described experimental conditions.
• Analyze the combined effect of spaceflight and DNA damage on fibroblast transcriptomes based on the bleomycin treatment protocol.
• Model cellular stress pathways activated by genotoxic agents in a low-gravity environment based on the described gene expression profiling.
• Compare spaceflight-induced gene expression signatures to terrestrial controls based on the experimental design.

Strengths

• Data originates from a controlled experiment conducted on the International Space Station by NASA.
• Experimental parameters are specified: cell type (AG01522), drug concentration (1.0 µg/ml bleomycin), and treatment duration (3 hours).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is stored in a BIN format, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration (NASA)

Collection Method

Microarray analysis of total RNA isolated from fibroblasts flown to the ISS, treated with bleomycin, and fixed in RNALater.

Time Range

The experiment was conducted after three days on the ISS, but the specific collection year is not provided.

Freshness

Last updated 2026-03-13 19:21:59.347005; freshness should be verified.

Geography

Data collected from the International Space Station (ISS).