Yeast Deletion Strains Fitness Profiles from Spaceflight and Ground Experiments

Approximately 10,700 yeast deletion strains, including ~4800 homozygous and ~5900 heterozygous strains, were cultured in microgravity and on Earth to quantify genetic requirements for survival. The genome-wide sensitivity profiles were generated by NASA, comparing growth in spaceflight versus ground conditions with and without osmotic stress. Results suggest spaceflight effects share mechanisms with DNA-damaging agents and redox state changes.

Use Cases

• Identify genes essential for survival in microgravity based on strain fitness comparisons.
• Model combined stressor effects by analyzing growth data with added hyperosmolar sodium chloride.
• Compare spaceflight-induced biological effects to drug mechanisms using similarity queries against a known drug compendium.
• Study the impact of redox state changes on cellular fitness using genome-wide sensitivity profiles.

Strengths

• Includes a complete collection of ~4800 homozygous and ~5900 heterozygous yeast deletion strains.
• Provides a quantitative, genome-wide assessment using molecular barcodes as unique strain identifiers.
• Compares multiple conditions: spaceflight vs. ground and with vs. without a secondary osmotic stressor.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Screening of molecularly barcoded yeast deletion collections cultured in custom hardware during spaceflight and ground control experiments.

Time Range

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Freshness

Last updated 2026-03-13 20:28:20.102901; freshness should be verified.

Geography

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