Genetic characterization of freshwater fishes using DNA barcoding with 12S rRNA gene from

Nine samples representing six fish species from rivers in Pakistan's Malakand division were sequenced for the 12S rRNA gene. The dataset includes genetic distance calculations, phylogenetic trees, and diversity indices, with sequences submitted to GenBank. Author Sohail Anjum published the data on figshare in April 2026.

Use Cases

• Validate species identification based on 12S rRNA gene sequences.
• Calculate genetic distances and diversity indices for phylogenetic analysis.
• Compare genetic variation within and between genera such as Carassius, Tor, Channa, and Garra.
• Construct phylogenetic trees using models like Maximum Likelihood (ML) and Neighbor-Joining (NJ).

Strengths

• Sequences are associated with GenBank accession numbers, providing traceability.
• Analysis includes specific genetic metrics: 509 singleton sites, 99 parsimony informative sites, and within-species K2P distances ranging from 0.000 to 0.00329.
• Data is published under a CC-BY-4.0 license, permitting open reuse.

Limitations

• The dataset is small, containing only nine samples from six species.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect geographic bias inherent to the specific rivers of the Malakand division.

Provenance

Source

figshare

Collection Method

Fish were sampled and identified morphologically; DNA was extracted from muscle tissue, PCR amplified, and sequenced.

Time Range

null

Freshness

Last updated 2026-04-13 09:28:51; freshness should be verified.

Geography

Rivers of the Malakand division, Pakistan.