Ginkgolic Acid Targets HSPA8 to Trigger Ferroptosis in Liver Cancer

A 2026 study by Ruohong Liu investigates the anticancer mechanism of Ginkgolic acid (GAC). The dataset likely contains results from integrated analyses including cell viability assays, RNA sequencing, drug affinity responsive target stability (DARTS), and validation assays like molecular docking and cellular thermal shift assays. Findings reveal GAC induces ferroptosis in hepatocellular carcinoma cells by directly targeting HSPA8 and promoting chaperone-mediated autophagy-dependent degradation of GPX4.

Use Cases

• Mechanistic validation of drug candidates based on DARTS and LC-MS/MS target identification.
• Analysis of ferroptosis biomarkers like GPX4 degradation and lipid peroxidation in cancer cell lines.
• Evaluation of chaperone-mediated autophagy activity using KFERQ reporter system data.
• Correlation of in vitro cell viability data with in vivo tumor growth suppression in an orthotopic mouse model.

Strengths

• Dataset is licensed under CC-BY-4.0, facilitating open reuse and sharing.
• Integrated multi-method validation includes genetic approaches (gene knockdown, mutant plasmids) and biophysical assays (CETSA, MST).
• Cross-platform presence on DataCite and Figshare signals importance and accessibility.

Limitations

• Column names and a precise row count are not provided, limiting understanding of data structure.
• Metadata completeness is medium; the dataset size is reported (2,919,837 bytes) but its composition is unclear.
• The dataset appears to be a static snapshot from a single 2026 study, lacking temporal or multi-study breadth.

Provenance

Source

Ruohong Liu, affiliated with Taylor & Francis.

Collection Method

Data generated from experiments using human HCC cell lines and an orthotopic HCC mouse model.

Freshness

Last updated 2026-03-25.