Grass Carp Catalase Gene Sequence and Functional Analysis

A 2263 bp cDNA sequence for the catalase gene (CiCAT) cloned from grass carp (Ctenopharyngodon idellus). The research by Xuyang Wang, published on figshare in 2026, investigates CiCAT's role in scavenging reactive oxygen species and inhibiting grass carp reovirus replication through autophagy.

Use Cases

• Analyze gene expression patterns in fish tissues based on the described tissue-wide constitutive expression of CiCAT.
• Study antiviral mechanisms in aquaculture species based on the described role of CiCAT in inhibiting GCRV replication.
• Investigate oxidative stress pathways in teleost fish based on the described function of CiCAT in scavenging ROS and resisting H₂O₂ and heavy metal stress.
• Explore subcellular protein localization based on the described co-localization of CiCAT with peroxisomes under H₂O₂ treatment.
• Model immune signaling and autophagy interactions based on the described mechanism linking ROS scavenging to mTORC1/ULK1 pathway activation.

Strengths

• The full-length cDNA sequence of CiCAT is precisely defined as 2263 bp with a 1575 bp ORF.
• Functional analysis includes specific experimental results: enhanced E. coli resistance to H₂O₂/heavy metals and inhibition of GCRV replication.
• The mechanistic investigation provides a detailed pathway linking ROS scavenging to immune suppression and autophagy.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (1.3 MB), likely containing only the research paper and supplementary files.

Provenance

Source

Xuyang Wang

Collection Method

Cloning and characterization of a gene from grass carp, followed by functional and mechanistic laboratory experiments.

Freshness

Last updated 2026-05-21 18:20:03; freshness should be verified.