FBXO31 E3 Ligase Degradation Data for FKBP12, Kinases, and BET Proteins

1.4 MB of experimental data on harnessing the FBXO31 E3 ligase for targeted protein degradation. The dataset, authored by Chenlu Zhang and last updated in April 2026, details the use of an amidated Ala-Phe motif to create degraders for targets including FKBP12, multiple kinases, and BET proteins BRD2, BRD3, and BRD4.

Use Cases

• Identify key residues in FBXO31 required for recruiter engagement based on mechanistic studies.
• Screen small-molecule binders for transformation into FBXO31-dependent degraders based on the described chemical recruiter strategy.
• Analyze ternary complex formation for FBXO31-mediated degradation based on the described experimental results.
• Compare degradation potency across protein targets like FKBP12, kinases, and BET proteins.

Strengths

• Data is shared under a specific open license (CC-BY-NC-4.0).
• The description provides concrete biological targets (FKBP12, kinases, BRD2/3/4) and a specific chemical motif (amidated Ala-Phe).
• File size is known (1.4 MB) and format is a standard spreadsheet (XLSX).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains experimental results from chemical and biological assays described in the summary.

Freshness

Last updated 2026-04-11 02:43:47; freshness should be verified.