Engineered Yeast β-Carotene Production Data from Xylose Fermentation

Liang Sun from the University of Illinois Urbana-Champaign engineered Saccharomyces cerevisiae yeast to produce β-carotene from xylose. The study reports a final titer of 772.81 mg/L β-carotene in a fed-batch bioreactor, a three-fold increase over production from glucose. The research explores the metabolic advantages of xylose as a carbon source for isoprenoid overproduction.

Use Cases

• Benchmarking yeast strain performance for β-carotene yield based on carbon source
• Analyzing the relationship between cytosolic acetyl-CoA pool size and product titer
• Studying gene expression levels (e.g., ACS1, HMG1) under different fermentation conditions
• Modeling metabolic fluxes in the mevalonate pathway when using xylose versus glucose

Strengths

• Reports a specific, high final product titer of 772.81 mg/L β-carotene
• Compares production performance across two carbon sources (xylose vs. glucose) with a reported three-fold improvement
• Identifies specific metabolic advantages (respiratory feature, acetyl-CoA pool, gene expression) from the fermentation profiling

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

University of Illinois Urbana-Champaign

Collection Method

Laboratory fermentation and metabolic profiling of genetically engineered yeast strains.