SARS-CoV-2 Spike Protein Expression Quantification for mRNA-LNP Vaccine Evaluation

An optimized immuno-multiple reaction monitoring method targets the SARS-CoV-2 spike fusion peptide SFIEDLLFNK. Ruben Almey published this dataset on figshare in 2026, detailing a workflow for quantifying antigen expression following mRNA or plasmid transfection. The method is described as offering high sensitivity, precision, and linearity across a broad dynamic range.

Use Cases

• Quantifying antigen expression levels based on the described immuno-MRM method targeting the spike fusion peptide.
• Profiling host cell proteomic responses based on the analysis of flow-through data mentioned in the description.
• Evaluating mRNA vaccine efficacy and safety based on the dual-function workflow for comprehensive characterization.
• Assessing protein translation in vitro based on the method's designed accuracy for antigen level measurement.

Strengths

• Method validated for high sensitivity, precision, and linearity as stated in the description.
• Dataset is associated with a specific, optimized immuno-MRM method targeting peptide SFIEDLLFNK.
• Workflow supports dual-function analysis of antigen levels and host cell responses.

Limitations

• Dataset size is 28.9 KB, indicating a very limited scope.
• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains experimental results from an optimized immuno-MRM laboratory method.

Freshness

Last updated 2026-04-23 11:04:24; freshness should be verified.