TraDIS Data for EHEC O157:H7 Virulence Gene Regulation

Transposon-directed insertion-site sequencing data identifies genes essential for adherence of the pathogenic Escherichia coli O157:H7 serotype. The dataset, authored by Miaomiao Liu and published in April 2026, includes results from a genome-wide screen for regulators of the LEE pathogenicity island. It comprises files in XLS, DOCX, and XLSX formats totaling 3.2 MB.

Use Cases

• Identify essential adherence genes from genome-wide TraDIS insertion data.
• Analyze HprR regulator binding to the hiuH promoter region for oxidative stress resistance.
• Correlate Ler-dependent regulation with expression levels of LEE pathogenicity island genes.
• Assess type III secretion system activity changes linked to specific gene disruptions.

Strengths

• Data originates from a genome-wide screen using transposon-directed insertion-site sequencing.
• Results define a regulatory framework for a major global public health pathogen.
• Dataset is openly available under a CC-BY-4.0 license.

Limitations

• Dataset size is 3.2 MB, indicating a relatively small volume of primary data.
• Specific row counts and column names for the tabular data are not provided.
• Data focuses on a single bacterial serotype, limiting generalizability to other pathogens.

Provenance

Source

figshare platform, authored by Miaomiao Liu.

Collection Method

Generated via transposon-directed insertion-site sequencing (TraDIS) genome-wide screen.

Freshness

Last updated in April 2026.