Fluorescent Aptamer Performance Data for 25-Hydroxyvitamin D3 Detection

Experimental data compares four truncated DNA aptamers for detecting 25-Hydroxyvitamin D3, a key marker of vitamin D status. The dataset likely contains affinity and specificity measurements for each aptamer variant under optimized conditions. The research was conducted by Lu Qiao at the Chinese Academy of Agricultural Sciences.

Use Cases

• Compare aptamer affinity based on truncation length and structural domain retention.
• Evaluate biosensor specificity for 25-Hydroxyvitamin D3 detection against other compounds.
• Optimize fluorescence detection protocols using PicoGreen dye and double-stranded DNA formation.
• Benchmark the limit of detection for novel aptamer-based assays.

Strengths

• Describes a specific, high-affinity aptamer (D3-4) with a reported limit of detection of 0.04μg/mL.
• Compares four distinct truncated aptamer variants derived from an original selection.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• Data may reflect experimental bias inherent to the specific laboratory setup described.

Provenance

Source

Lu Qiao, Chinese Academy of Agricultural Sciences

Collection Method

Experimental comparison of truncated DNA aptamers using fluorescence intensity changes.