HPLC-DAD Analysis of Alkaloids in Mexican Prickly Poppy Plant Tissues

Jorge F. Xool-Tamayo presents an HPLC-DAD method for analyzing benzylisoquinoline alkaloids in Argemone mexicana. The method quantifies berberine, chelerythrine, and sanguinarine with linearity between 0.5-20 µg mL-1, detection limits as low as 0.05 µg mL-1, and intra-day variation below 0.49%. It was validated on field-collected plant tissues and fulfills United States Pharmacopeia system suitability criteria.

Use Cases

• Validate HPLC-DAD methods for alkaloid quantification based on described system suitability tests.
• Quantify berberine, chelerythrine, and sanguinarine in plant extracts based on the described calibration ranges.
• Compare alkaloid content across different tissues of Argemone mexicana based on the analysis of field-collected plants.
• Assess method reproducibility for plant metabolite analysis based on reported recovery rates higher than 95%.

Strengths

• Method validation includes specific linearity ranges (0.5-20 µg mL-1) and low limits of detection (e.g., 0.05 µg mL-1 for sanguinarine).
• High reproducibility indicated by intra- and inter-day variation values < 0.49% and alkaloid recoveries higher than 95%.
• System suitability tests, including resolution and tailing factor, were performed according to United States Pharmacopeia standards.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Jorge F. Xool-Tamayo via paperswithcode

Collection Method

HPLC-DAD analysis of field-collected Argemone mexicana plant tissues.

Geography

Likely Mexico, inferred from the plant species Argemone mexicana (Mexican prickly poppy).