Huntingtin-HAP40 Structural Data: Cryo-EM Maps, SAXS Profiles, and Ensemble Models

Supplementary Data files accompany the manuscript by Harding et al. on Huntingtin structure orchestrated by HAP40. The collection includes 11 supplementary data files, such as multiple sequence alignments, cryo-EM maps, SAXS profiles, and ensemble models for different Huntingtin variants. The data was produced by Rachel Harding at the University of Toronto.

Use Cases

• Consurf analysis based on multiple sequence alignments for HTT and HAP40
• Structural modeling based on cryo-EM maps and SAXS profiles for HTT-HAP40 complexes
• Cross-linking mass spectrometry (XL-MS) data analysis for protein-protein interaction validation
• Ensemble modeling and weighting analysis for different Huntingtin polyglutamine lengths

Strengths

• Includes 11 distinct supplementary data files covering multiple experimental techniques
• Provides data for three specific Huntingtin variants (Q23, Q54, Δexon 1) enabling comparative analysis
• Data is directly linked to a peer-reviewed scientific manuscript, providing context

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count and file sizes are unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

University of Toronto (Rachel Harding)

Collection Method

Experimental data from cryo-EM, SAXS, and XL-MS techniques, generated as supplementary material for a research paper.