Hybrid Enterobacteriaceae Assemblies: PacBio+Illumina vs ONT+Illumina

158 hybrid genome assemblies for 20 bacterial isolates compare PacBio and Oxford Nanopore long-read technologies combined with Illumina short reads. Assemblies were produced using the Unicycler tool with four distinct long-read preparation strategies: basic, corrected, filtered, and subsampled. The data supports a 2019 study by De Maio, Shaw, and the REHAB consortium on the accuracy of long-read sequencing methods for complex bacterial genomes.

Use Cases

• Benchmarking hybrid assembly performance based on different long-read preparation strategies described in the study
• Comparing the accuracy of PacBio and Oxford Nanopore long-read sequencing platforms for bacterial genomics
• Developing or validating genome assembly pipelines using a dataset with multiple assembly versions per isolate

Strengths

• Includes 158 hybrid assemblies across 20 bacterial isolates, enabling comparative analysis
• Each isolate has assemblies from four distinct long-read preparation strategies, allowing method comparison
• Data is associated with a peer-reviewed preprint (biorxiv 530824) providing methodological context

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

Liam P. Shaw and the REHAB consortium

Collection Method

Hybrid genome assembly using Unicycler with Illumina and long-read (PacBio/ONT) sequencing data