Bacillus Pseudomycoides Genomic Distance and dDDH Results

Results from a 2026-01-30 analysis by the Type (Strain) Genome Server (TYGS) provide digital DNA-DNA hybridization (dDDH) values and intergenomic distances for Bacillus pseudomycoides strain CHAES I 2_2. The data includes comparisons against ten closest type strains determined via MASH and GBDP algorithms. Author Alina Kharchuk published the findings on figshare in April 2026.

Use Cases

• Calculate species and subspecies clustering using the 70% and 79% dDDH thresholds provided in the analysis.
• Infer phylogenetic relationships using the GBDP-derived intergenomic distances and the balanced minimum evolution tree method described.
• Validate taxonomic placement by comparing MASH distances and 16S rDNA BLAST bitscores against the ten closest type strain genomes.
• Assess branch support in phylogenetic trees using the 100 pseudo-bootstrap replicates generated for distance calculations.

Strengths

• Data is sourced from the authoritative Type (Strain) Genome Server (TYGS) platform.
• Analysis employs multiple established methods: MASH, GBDP under 'coverage' and 'trimming' algorithms, and FASTME 2.1.6.1.

Limitations

• Dataset is very small at 9.8 KB, indicating limited scope likely to a single strain's comparison results.
• Specific row count, column names, and sample data are unavailable, hindering immediate analysis.
• Temporal coverage is a single snapshot from 2026-01-30, with no historical or multi-timepoint data.

Provenance

Source

Type (Strain) Genome Server (TYGS)

Collection Method

Whole genome-based taxonomic analysis using MASH, GBDP, and FASTME on uploaded genome sequence data.

Time Range

Analysis date: 2026-01-30

Freshness

Analysis was performed on 2026-01-30 and published in April 2026, representing very recent computational results.