Cryo-Electron Tomography Data on ER-Phagophore Membrane Continuity in Drosophila

Yufei Huang published a 399.4 MB collection of cryo-electron tomography and volume electron microscopy data on April 8, 2026. It captures the in situ architecture of mitophagosome formation in the developing Drosophila intestine. The dataset reveals direct membrane continuity between the endoplasmic reticulum and the phagophore during mitochondrial clearance.

Use Cases

• Reconstruct 3D cellular architecture from cryo-electron tomography (cryo-ET) and volume electron microscopy data to visualize ER-phagophore connections.
• Analyze membrane continuity phenotypes in Vps13D mutant enterocytes compared to wild-type samples.
• Train segmentation models to identify mitochondria, endoplasmic reticulum, and phagophore structures within the volumetric image data.
• Validate biophysical models of membrane expansion during mitophagy using the empirical membrane continuity evidence.

Strengths

• Data combines multiple high-resolution imaging techniques: cryo-ET, SOLIST, cryo-FIB milling, and volume electron microscopy.
• Dataset size is 399.4 MB, indicating a substantial volume of imaging data.
• Specific biological context is provided, focusing on mitophagy in Drosophila intestine during development.

Limitations

• Sample data and specific row/column counts for analysis are unavailable.
• Data is contained in a PPTX file format, which is non-standard for raw scientific image data and may require extraction.
• Biological scope is narrow, focused on a specific process (mitophagy) in a single model organism (Drosophila).

Provenance

Source

Yufei Huang via figshare.

Collection Method

Data gathered using cryo-electron tomography, serialized on-grid lift-in sectioning for tomography (SOLIST), cryo-focused ion beam milling, and volume electron microscopy.

Freshness

Last updated on April 8, 2026.