Borrelia burgdorferi RNA Chaperone Activity Data from Gradient Profiling

Gradient profiling identified five proteins in the Lyme disease spirochete Borrelia burgdorferi exhibiting RNA chaperone activity. The dataset includes results for hypothetical proteins BB0749, BB0713, BB0796, CheY2, and FlgV, demonstrating RNA unwinding, annealing, and strand displacement. Author Taylor Van Gundy published the data on figshare under a CC-BY-4.0 license, last updated June 3, 2026.

Use Cases

• Validate RNA-binding protein predictions based on gradient profiling co-sedimentation data.
• Study RNA structural remodeling mechanisms based on the described unwinding and annealing activities.
• Investigate the role of hypothetical proteins (BB0749, BB0713, BB0796) in Borrelia burgdorferi gene regulation.
• Analyze the RNA-binding functions of chemotaxis (CheY2) and flagella-associated (FlgV) proteins.

Strengths

• Includes data for five specific proteins (BB0749, BB0713, BB0796, CheY2, FlgV) with confirmed RNA chaperone activity.
• In vivo co-IP assays demonstrate RNA binding for BB0749 within B. burgdorferi.
• Data files are provided in multiple formats (PDF, DOCX, XLSX, TIF) for different analysis needs.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Gradient profiling to identify proteins co-sedimenting with small RNAs and nascent mRNAs.

Freshness

Last updated 2026-06-03 13:40:06; freshness should be verified.