Trifolium repens Glycyrrhetinic Acid Biosynthesis Data

Metabolomic and transcriptomic data elucidates the glycyrrhetinic acid biosynthetic pathway in the legume Trifolium repens. The dataset includes results from heterologous reconstruction in yeast, identifying key enzymes and achieving a titer of 3.07 mg/L. It was authored by Kangxin Hou and last updated on May 12, 2026.

Use Cases

• Identify conserved cytochrome P450 genes for triterpenoid synthesis based on phylogenetic analyses mentioned in the description
• Optimize yeast chassis for heterologous production based on cytochrome P450 reductase screening results
• Compare triterpenoid accumulation across plant tissues based on targeted metabolomics data
• Reconstruct metabolic pathways in non-model legumes based on identified gene functions

Strengths

• Includes experimental validation via heterologous reconstruction in yeast, confirming gene function
• Reports a specific production titer of 3.07 mg/L glycyrrhetinic acid in the engineered yeast strain
• Identifies specific enzyme classes: three β-amyrin synthases and three cytochrome P450s

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data may reflect the specific experimental conditions and plant tissues inherent to the source study

Provenance

Source

figshare

Collection Method

Generated from targeted metabolomics, transcriptomic analyses, and heterologous reconstruction experiments.

Freshness

Last updated 2026-05-12 10:32:18; freshness should be verified