iGluSnFR Wide-Field Glutamate Imaging of Mouse V1 Under 2-72 Hz Flicker

Widefield glutamate imaging data from awake mice V1 during 2-72 Hz flicker stimulation, recorded at a 150 Hz sampling rate. Created by Rasa Gulbinaite and published via Borealis Dataverse in 2026, the dataset captures cortical responses to 10-second flicker trials across a 128x128 pixel field of view.

Use Cases

• Modeling glutamate kinetics using the 150 Hz sampling rate to track neurotransmitter clearance
• Identifying resonance peaks at 8, 15, and 33 Hz within the V1 tuning curves
• Validating linear wave equations using the 128x128 pixel spatial activity patterns to identify standing waves

Strengths

• 150 Hz sampling rate for high temporal resolution
• 128x128 pixel spatial resolution at 67 μm per pixel
• Covers a broad flicker frequency range from 2 to 72 Hz

Limitations

• Unknown total number of trials or subjects
• Geographic and biological bias inherent to mouse models
• Potential for residual hemodynamic signal noise despite 1 mm focal depth

Provenance

Source

Rasa Gulbinaite, Borealis Harvested Dataverse

Collection Method

In vivo widefield glutamate imaging using a CCD camera and iGluSnFR reporter in awake head-fixed mice

Freshness

Last updated February 2026