Illumina NGS RNA-seq and RRBS Sequencing on Spaceflight Mouse Retina

NASA's Rodent Research-1 project collected retinal tissue from C57 BL/6J mice after a 37-day mission on the International Space Station. The dataset contains Illumina NGS RNA-seq and RRBS sequencing data to investigate spaceflight-associated neuro-ocular syndrome (SANS). It was last updated in March 2026.

Use Cases

• Identify gene expression changes in retinal tissue based on transcriptomic sequencing data.
• Analyze epigenetic modifications related to spaceflight stress based on methylomic RRBS data.
• Model biological pathways affected by microgravity based on combined transcriptomic and methylomic profiles.
• Compare spaceflight-induced molecular changes to ground control models for SANS research.

Strengths

• Data originates from a controlled NASA spaceflight experiment with a defined 37-day mission duration.
• Focuses on a specific tissue (retina) and a well-defined mouse model (C57 BL/6J) for studying SANS.
• Employs two high-throughput sequencing approaches (RNA-seq and RRBS) for multi-omics analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and dataset scale are unknown, which may limit suitability assessment.
• Data is provided in a BIN format, which may require specialized tools for access and interpretation.

Provenance

Source

National Aeronautics and Space Administration (NASA)

Collection Method

Illumina next-generation sequencing (RNA-seq and Reduced Representation Bisulfite Sequencing) on retinal tissue from spaceflight mice.

Time Range

Experiment conducted during the Rodent Research-1 mission; specific collection dates not provided.

Freshness

Last updated 2026-03-13 19:48:32.437728; freshness should be verified.

Geography

Samples from mice housed on the International Space Station (ISS).