TbGOS02: Fluorescent and Brightfield Images of Trypanosoma Brucei Cells

120 images captured using a novel Fluorescence in Live Trypanosome (FILT) setup, comprising 60 fluorescent and 60 brightfield frames. The dataset includes images of a field of 88 cells and cropped sequences for four individual cells. Author Yanika Borg's work, referenced in a 2015 thesis and a 2021 study, is linked to this data.

Use Cases

• Analyze fluorescence intensity over time based on the 60-frame sequences for individual cells.
• Compare cellular morphology between fluorescent and brightfield imaging modalities.
• Train image segmentation models to identify and isolate individual trypanosome cells from a field of view.

Strengths

• Includes 120 total images (60 fluorescent, 60 brightfield) for a field of 88 cells.
• Provides detailed cropped image sequences for four specific, enumerated cells (08, 17, 63, 76).
• Data is directly linked to specific, cited research methodology (Borg 2015, Borg et al. 2021).

Limitations

• Data is packaged in PDF files, which is an atypical and potentially cumbersome format for image analysis workflows.
• The dataset scale is limited to images from a single experimental setup and cell line.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

Yanika Borg, research associated with University College London.

Collection Method

Images captured using a Fluorescence in Live Trypanosome (FILT) experimental setup.