Immunofluorescence Images of Mouse and Opossum Visual Cortex

Ryan Gorzek's dataset contains immunofluorescence images from the primary visual cortex of adult mouse and gray short-tailed opossum. The images, generated for a 2026 comparative study, include stains for parvalbumin, WFA, NeuN, and CC1 to support analysis of cortical cell types and inhibitory circuitry.

Use Cases

• Quantify parvalbumin-positive cell density across medial and lateral V1 regions in mouse and opossum sections.
• Analyze co-localization of Wisteria floribunda agglutinin (WFA) staining with parvalbumin immunoreactivity to study peri-neuronal nets.
• Use NeuN-stained images to estimate total neuronal density for comparative anatomical studies between species.
• Segment and classify oligodendrocytes in opossum sections using CC1 immunofluorescence markers.

Strengths

• Includes images from two deeply diverged mammalian species (mouse and opossum) for comparative analysis.
• Covers multiple biological markers: parvalbumin, WFA, NeuN, and CC1 across different stain combinations.
• Images are provided as raw or minimally processed TIFF/TIF files, supporting reproducibility and reanalysis.
• Dataset is associated with a specific 2026 publication (Gorzek & Trachtenberg) detailing the study methodology.

Limitations

• The dataset is relatively small at 86.1 MB, limiting the scale for training large deep learning models.
• Sample size and exact number of images (rows) are unknown, making it difficult to assess statistical power.
• Data is restricted to coronal sections of the primary visual cortex, not representing whole-brain or other cortical areas.

Provenance

Source

figshare, author Ryan Gorzek.

Collection Method

Immunofluorescence imaging of coronal brain sections stained for specific markers.

Time Range

Associated with study dated 2026; specific collection period unknown.

Freshness

Last updated March 13, 2026.

Geography

Laboratory origin; species are Mus musculus (mouse) and Monodelphis domestica (opossum).