Human Serum Haptoglobin Purification Images for Glycoproteomic Studies

A figshare dataset by In-Seok Yeo, last updated May 14, 2026, detailing a two-step purification method for haptoglobin. The 1.3 MB dataset contains TIF image files, likely documenting experimental results from CBB staining, western blotting, and lectin blotting analyses. The work aims to provide a reliable approach for preparing functionally intact, immunoglobulin-free haptoglobin.

Use Cases

• Validate protein purification protocols based on the described two-step immunoglobulin depletion and affinity column method.
• Analyze glycoprotein purity and contamination based on the CBB staining, western blotting, and lectin blotting results mentioned in the description.
• Study haptoglobin glycan structures based on the LC–MS/MS analysis referenced in the description.
• Assess ligand-binding functionality of purified proteins based on the hemoglobin binding assays described.

Strengths

• Dataset is licensed under CC-BY-4.0, permitting broad reuse with attribution.
• Methodology is described in detail, including specific techniques like thiophilic adsorption and anti-haptoglobin affinity columns.
• Functional validation of the purified haptoglobin via hemoglobin binding assays is documented.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (1.3 MB), which may limit the scope of analysis.

Provenance

Source

figshare

Collection Method

Experimental data from a two-step purification strategy involving thiophilic adsorption and immunoaffinity chromatography, analyzed via staining, blotting, and LC–MS/MS.

Time Range

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Freshness

Last updated 2026-05 14 06:54:40; freshness should be verified.

Geography

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