Gene Expression Biodosimetry: Radiation Dose Models from Human Blood

10 healthy donors provided peripheral blood and AHH-1 cells irradiated with Cobalt-60 γ-rays at doses from 0 to 8 Gy. The dataset contains gene expression patterns for candidate biomarkers ZMAT3, SESN1, AEN, and TRIAP1, measured 6–48 hours post-irradiation, used to build and validate multi-gene calibration models for dose prediction. These models achieved high predictive accuracy (R² = 0.81–0.89) and were validated with independent ex vivo and in vivo irradiated samples.

Use Cases

• Validating transcriptional biomarkers for biological dosimetry based on dose-dependent gene expression patterns.
• Building multi-gene regression models for radiation dose estimation using ΔCt values.
• Assessing the impact of dose rate (0.2–2 Gy/min) on gene expression signatures.
• Comparing ex vivo and in vivo model performance for point-of-care application development.

Strengths

• Includes data from a controlled irradiation study with 10 human donors and multiple dose points (0, 1, 2, 4, 6, 8 Gy).
• Models were validated on independent cohorts, achieving high predictive accuracy (R² = 0.81–0.89).
• Gene expression was characterized across a 6–48 hour post-irradiation time window, providing temporal dynamics.

Limitations

• Column names and exact row count are not provided, limiting understanding of data structure.
• The dataset description is truncated in the provided metadata.
• The primary data format (TIF, DOC) suggests supplementary files; core analysis data may be in other formats.

Provenance

Source

Experimental study using human B lymphoblastoid cells (AHH-1) and peripheral blood from healthy donors.

Collection Method

Cells and blood were irradiated with Cobalt-60 γ-rays; gene expression was analyzed via qPCR or similar methods to establish calibration curves.

Time Range

Post-irradiation measurements taken from 6 to 48 hours.

Freshness

Last updated 2026-03-19.

Geography

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