Barley Gene Expression Data for Thermotolerance Analysis

An RNA-seq and qRT-PCR dataset from a study on transgenic barley overexpressing the TaHsfA6b heat shock factor gene from wheat. The data shows upregulated expression of heat shock proteins, chaperonins, DNAJ, LEA proteins, and anti-oxidative enzyme genes in transgenic lines compared to wild-type plants under heat stress. The dataset was published by Anuj Kumar Poonia in an open-access paper.

Use Cases

• Analyze heat shock protein (HSP) gene expression patterns based on RNA-seq data mentioned in the description
• Compare reactive oxygen species (ROS) homeostasis mechanisms between transgenic and wild-type plants based on the described measurements
• Study the regulatory network of the TaHsfA6b gene based on its effect on abiotic stress-responsive genes
• Investigate the phenotypic effects of gene overexpression on thermotolerance in barley

Strengths

• Dataset is derived from RNA-seq and qRT-PCR analysis, suggesting quantitative gene expression measurements.
• The study reports significant improvement in thermotolerance and no negative phenotypic changes in transgenic lines, providing clear experimental outcomes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Anuj Kumar Poonia

Collection Method

Laboratory experiment involving transgenic barley lines, RNA-seq, and qRT-PCR analysis.