In Vitro Bioassay Evidence for Chemical Mixtures from Environment to Humans

Complex organic chemical mixtures extracted from environmental samples, food items, and human blood were tested in 22 in vitro bioassays targeting neurodevelopmental and reproductive health pathways. The dataset, authored by Beate Escher and last updated in June 2026, contains results from assays including neurite outgrowth inhibition and androgen receptor antagonism. Experimental mixture effects of 24 quantified chemicals were compared to concentration addition models.

Use Cases

• Modeling mixture toxicity based on concentration addition principles described in the study.
• Identifying bioactive contaminants in the food chain using the described bioassay endpoints.
• Assessing environmental and human health risks using preliminary effect-based trigger (EBT) values mentioned for water and food.
• Investigating the contribution of unannotated molecular features to total bioactivity as indicated by nontarget analysis.

Strengths

• Data covers 22 distinct in vitro bioassays targeting specific health pathways.
• Analysis includes 24 quantified chemicals and their mixture effects.
• Samples were taken from multiple matrices including wastewater, surface water, fish, milk, and human blood.
• File size is 2.8 MB, indicating a focused and manageable dataset.

Limitations

• Row count and specific column definitions are unknown, requiring manual inspection after download.
• The extraction method is described as having a bias toward more hydrophilic chemicals over highly hydrophobic ones.
• The dataset's primary format is XLSX, which may require specific tools for programmatic analysis.

Provenance

Source

figshare, authored by Beate Escher.

Collection Method

Organic chemicals were extracted from environmental, food, and human blood samples and tested in bioassays.

Freshness

Last updated 2026-06-02 12:36:22.