Integrative Transcriptomics and Proteomics Profiling of Arabidopsis thaliana Elucidates No

NASA's dataset provides integrative transcriptomics and proteomics profiling of Arabidopsis thaliana seedlings grown aboard the International Space Station. The data captures molecular responses to spaceflight stressors like microgravity and radiation, with samples collected at 10 days old. Analysis suggests alterations in photosynthetic machinery, cell wall biosynthesis, and metabolic pathways.

Use Cases

• Modeling plant stress response pathways based on coordinated transcriptome and proteome data.
• Investigating organ-specific shifts in photosystem core-regulatory proteins under microgravity.
• Analyzing changes in cellulose synthase and cell wall polysaccharide biosynthesis gene expression in spaceflight.
• Studying alterations in ribosome, spliceosome, and proteasome activity abundance from spaceflight samples.
• Examining polar auxin redistribution and intracellular transport mechanisms under spaceflight conditions.

Strengths

• Data integrates RNA-seq transcriptomics and TMT-labeled LC-MS/MS proteomics for a multi-omics view.
• Samples were germinated and grown in microgravity aboard the International Space Station, providing a unique environment.
• Research provides specific insights into altered abundances of proteins like CELLULOSE SYNTHASES and TUBULIN COFACTOR B.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is in BIN format, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Arabidopsis seedlings were germinated and grown in NASA BRIC LED hardware on the ISS, with ground controls on Earth. At 10 days old, seedlings were frozen in RNA-later, returned to Earth, and analyzed via RNA-seq and LC-MS/MS.

Time Range

Sample collection occurred when seedlings were 10 days old.

Freshness

Last updated 2026-03-13 20:00:15.986324; freshness should be verified.

Geography

Samples from the International Space Station (microgravity) and Earth (ground control).