Mouse Intestinal ILC3 Cytokine Quantification in Necrotizing Enterocolitis Model

Experimental data from a mouse study on necrotizing enterocolitis (NEC) investigates the role of autophagy in group 3 innate lymphoid cells (ILC3s). The dataset contains flow cytometry measurements quantifying IL-17A and IL-22 production in total and double-negative ILC3 subsets across four experimental groups, with 6 biological replicates per group. Author Junyu He published the 2.8 GB dataset on figshare in April 2026 under a CC-BY-4.0 license.

Use Cases

• Compare cytokine production (IL-17A, IL-22) between autophagy-intact and autophagy-deficient ILC3s based on the described mouse genotypes.
• Assess the effect of metabolic interventions (lactate, etomoxir) on ILC3 effector function in a disease model as outlined in the experimental groups.
• Analyze differences in immune cell subsets (total ILC3s vs. double-negative ILC3s) within the intestinal lamina propria.
• Validate statistical findings from the described one-way ANOVA with Tukey's post-hoc test using the underlying flow cytometry data.

Strengths

• Includes 6 biological replicates per experimental group, providing a basis for statistical analysis.
• Experimental design is clearly described with four distinct groups for comparison.
• Data was acquired using a specific instrument (CytoFLEX S flow cytometer) and a detailed antibody panel is listed.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Partial data were excluded from the repository; all underlying data are available only upon request.
• Row count is unknown, which may limit suitability assessment for certain analyses.

Provenance

Source

figshare

Collection Method

Flow cytometry performed on intestinal lamina propria cells from genetically modified C57BL/6 mice subjected to experimental NEC induction.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-16 02:17:23; freshness should be verified.

Geography

Laboratory study; geographic origin of data not specified.