GPER Subcellular Localisation and P16L Variant Genotyping Data

481.8 MB of experimental records, raw data files, and analytical outputs for a master's dissertation investigating G protein-coupled oestrogen receptor localisation. The dataset includes molecular cloning validation data, confocal microscopy images from transfected HEK293T cells, and RFLP genotyping data from a South African cohort. It was authored by Tlotlo Matladi and last updated on May 7, 2026.

Use Cases

• Analyze subcellular localisation patterns based on confocal microscopy images of wild-type and chimeric GPER constructs
• Validate molecular cloning constructs based on plasmid maps, primer records, and Sanger sequencing chromatograms
• Determine genotype prevalence based on Restriction Fragment Length Polymorphism (RFLP) data from a South African cohort

Strengths

• Includes primary, unedited data files such as Chemidoc-generated gel images and confocal microscopy scans
• Contains comprehensive experimental records covering cloning, imaging, and genotyping for a single dissertation project
• Files are provided in multiple formats (PDF, SCN, XLSX, TIF, PNG, CZI) suitable for different analysis tools

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Data may reflect geographic bias inherent to the specific South African cohort studied

Provenance

Source

figshare

Collection Method

Generated as part of a master's dissertation project involving molecular cloning, cell imaging, and genetic analysis.

Freshness

Last updated 2026-05-07 06:00:01; freshness should be verified

Geography

Includes data from a South African cohort.