Mouse Pancreatic Islet Proteome Under HFSC Diet

A proteomic analysis of pancreatic islets from male C57BL/6J mice fed a high-fat, simple-carbohydrate diet for 150 days. The dataset contains 386 identified proteins, with 30 differentially expressed proteins identified after statistical filtering. It was uploaded to figshare by Vijayalakshmi Gangadhara on May 5, 2026.

Use Cases

• Identify differentially expressed proteins in pancreatic islets based on HFSC diet exposure.
• Analyze upregulated pathways related to cytoplasmic translation and ribosomal biogenesis.
• Investigate downregulated carbohydrate and lipid metabolic pathways.
• Study stress-responsive proteins like MANF and HSP90B1 for ER stress evidence.
• Validate proteomic evidence of molecular adaptations associated with metabolic stress.

Strengths

• Dataset includes 386 identified proteins from pancreatic islets.
• 30 differentially expressed proteins were identified with statistical thresholds (fold change ≥ 1.5, FDR-adjusted q-value ≤ 0.05).
• Functional enrichment analysis revealed significant pathway upregulation and downregulation.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a specific mouse strain (C57BL/6J) and gender (male), limiting generalizability.

Provenance

Source

figshare

Collection Method

Label-free ESI-nanoLC-MS/MS proteomic profiling of collagenase-isolated pancreatic islets.

Time Range

Experimental duration of 150 days.

Freshness

Last updated 2026-05-05 17:03:32; freshness should be verified.