Experimental Data for pH-Mediated SNV Detection on Double-Stranded DNA

Yu Gong developed a physicochemical strategy for detecting single-nucleotide variants (SNVs) on double-stranded DNA. The dataset, shared on figshare in April 2026, contains experimental results for a method that uses pH-mediated toehold-controlled ligation. The workflow comprises two steps and achieves detection within 45 minutes.

Use Cases

• Benchmarking rapid SNV detection methods based on the described 45-minute workflow.
• Developing instrument-free diagnostic tools based on the pH-mediated dsDNA unwinding principle.
• Optimizing probe assembly and ligation efficiency for mutant allele recognition as described in the method.
• Training models for signal interpretation from the in vitro transcription reaction output mentioned in the description.

Strengths

• The dataset is associated with a method that achieves detection and readout within 45 minutes.
• The described workflow comprises only two operational steps.
• The dataset is small (10.6 KB), facilitating quick download and inspection.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's scope is limited to the specific experimental results of the described method.

Provenance

Source

figshare

Collection Method

Experimental results from a physicochemical detection strategy.

Time Range

Unknown

Freshness

Last updated 2026-04-28 00:13:26; freshness should be verified.

Geography

Unknown