ATP1 Probe Data: Autophagic Flux Quantification in Live Cells and Mice

The ATP1 dataset documents the design and application of an activity-based autophagy probe for dynamically quantifying autophagic flux. The data, authored by Yaping Lu and last updated in May 2026, includes a 45.6 MB DOCX file describing the probe's development and performance in live cells and wild-type mice. The probe displays LC3-binding-triggered fluorogenic activation and autophagosome-lysosome fusion-triggered ratiometric changes.

Use Cases

• Developing non-transfection-based assays for tracking autophagic flux based on the probe's LC3-binding specificity.
• Quantifying dynamic signal changes in live cells based on the probe's ratiometric fluorescence behavior.
• Screening for autophagy modulators in drug discovery based on the probe's real-time tracking capability.
• Validating autophagy assays in primary cells and wild-type mice where transfection-based methods are impractical.

Strengths

• The probe is described as having low background signals and compatibility with primary cells.
• The dataset is licensed under CC-BY-4.0, allowing for open sharing and adaptation.
• The probe's performance is validated in live cells and mice, with a dynamic signal paralleling the mRFP-GFP-LC3 assay.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a 45.6 MB DOCX file, which may require specific software for access and lacks a structured data format.

Provenance

Source

Yaping Lu via figshare

Collection Method

Iterative, docking-guided design and synthesis of a fluorescent probe, with application in live cells and mice.

Freshness

Last updated 2026-05-21 05:40:05; freshness should be verified.