Mouse Brain Lipidomics After VPS13A Knockdown, 172 Lipid Species

Targeted LC-HRMS lipidomics data from mouse brain tissue investigates lipid metabolic alterations associated with neuronal VPS13A deficiency. The dataset quantifies 172 lipid species across 17 classes from pooled frontal cortex and striatum samples of 12 mice. It was created by Gisela Besa-Selva and published on figshare in April 2026.

Use Cases

• Identify lipid class alterations based on quantified levels of 172 lipid species.
• Investigate early lipid homeostasis changes based on data from VPS13A knockdown versus control mice.
• Correlate lipidomic profiles with neuronal dysfunction based on samples from specific brain regions (frontal cortex and striatum).
• Validate lipid transfer protein function based on lipid species quantified using class-specific internal standards.

Strengths

• Quantifies 172 specific lipid species across 17 lipid classes.
• Includes data from 12 mice (6 knockdown and 6 control), balanced by sex.
• Lipid levels are normalized to protein content (pmol/mgprot) using internal standards.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a pooled sample of two brain regions, limiting individual animal-level analysis.

Provenance

Source

figshare, author Gisela Besa-Selva.

Collection Method

Lipidomic profiling via LC-HRMS on tissue after stereotaxic injection of AAV8 vectors for VPS13A-specific shRNA knockdown.

Freshness

Last updated 2026-04-23 09:32:01; freshness should be verified.