Lecanemab: Microglial Program Analysis in Alzheimer's Disease

A 15.4 MB computational re-analysis repository accompanies a manuscript submitted to Glia. It contains code, tables, and figures analyzing two publicly deposited datasets (GSE297665, GSE297667) from Albertini et al., Nature Neuroscience, 2025. The master R script reproduces an analytical pipeline for scRNA-seq and spatial transcriptomic data, including differential expression and pathway enrichment.

Use Cases

• Reproduce differential expression analysis across plaque-distance zones based on the described pseudobulk edgeR method.
• Perform Reactome pathway enrichment analysis based on the fgsea method described.
• Score microglial states against published taxonomies (Sun 2023, Green 2024) based on the described scoring method.
• Analyze Fc-dependent spatial amplification comparing Lecanemab and LALA-PG based on the described analysis.
• Generate publication figures (Figures 1-5 and supplementary) based on the provided R script.

Strengths

• Includes a master R script that reproduces the full analytical pipeline described in six steps.
• Contains curated reference tables, differential expression results, pathway results, sensitivity analyses, and all manuscript figures.
• All input data are publicly available from NCBI GEO, facilitating verification and extension.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Third-party computational re-analysis of datasets GSE297665 and GSE297667 from Albertini, Zielonka, De Strooper et al., Nature Neuroscience, 2025.

Collection Method

No new experimental work; analysis includes ingestion of scRNA-seq and spatial transcriptomic data, pseudobulk differential expression, pathway enrichment, microglial state scoring, and spatial amplification analysis.

Freshness

Last updated 2026-05-25 17:58:55; freshness should be verified.