Norovirus Antibody Binding And Neutralization Profiles

LIBRA-seq analysis maps 25 monoclonal antibodies from healthy human blood donors, linking receptor sequences to antigen specificity. Binding breadth and functional neutralization data are provided for antibodies tested against a panel of norovirus genotypes, including pandemic GII.4 and emerging GII.17. The dataset was authored by Perry Wasdin and published in April 2026.

Use Cases

• Predict antibody cross-reactivity across norovirus genotypes using B-cell receptor sequence features.
• Classify neutralizing versus non-neutralizing antibodies based on binding profiles and HBGA blocking assay results.
• Identify shared antigenic targets by analyzing epitope competition assay data for overlapping binding sites.
• Train models to link B-cell receptor sequences from LIBRA-seq to specific antigen reactivity profiles.

Strengths

• Single-cell resolution data for antigen-specific B cells.
• 25 monoclonal antibodies with characterized binding and functional data.
• Binding analyses cover multiple norovirus genotypes, including GII.4 and GII.17.

Limitations

• Sample size is limited to antibodies from healthy adult blood donors, not infected or vaccinated individuals.
• The dataset focuses on a specific viral domain (P domain), not full virion responses.
• Neutralization data is derived from a surrogate HBGA blocking assay, not live virus assays.

Provenance

Source

Perry Wasdin via figshare.

Collection Method

Linking B cell Receptor to Antigen Specificity through Sequencing (LIBRA-seq) applied to peripheral blood mononuclear cells from human blood donors.

Freshness

Last updated in April 2026.