Human Heart Single-Nucleus RNA-Seq Atlas Across Lifespan

Single-nucleus RNA sequencing data from 442,239 nuclei across 54 non-failing human heart tissues. The atlas covers 29 individuals from development through aging, including left and right ventricles. Hao Jia published this resource on figshare in April 2026.

Use Cases

• Model cardiac transcriptomic age using single-nucleus expression profiles to predict chronological age or detect accelerated aging signatures.
• Analyze cell type-specific transcriptional trajectories, such as cardiomyocyte states enriched for senescence- and disease-related gene signatures.
• Identify regulatory network dynamics, like the activity of the PRDM16 transcription factor, and correlate its decline with age in cardiomyocytes.
• Compare gene expression homeostasis and inflammatory signaling patterns across major cell types (e.g., cardiomyocytes, fibroblasts) over the lifespan.

Strengths

• Large-scale data with 442,239 single-nucleus profiles enabling detailed cellular analysis.
• Covers a wide biological range with 54 tissue samples from 29 individuals across development, adulthood, and aging.
• Includes matched left and right ventricle samples, allowing for chamber-specific comparisons.

Limitations

• Limited to non-failing heart tissues, lacking direct comparison with a large set of diseased hearts.
• Sample size of 29 individuals may limit power for detecting rare cell states or sub-populations.
• Data is from a single study, so findings may require validation in independent cohorts.

Provenance

Source

figshare, authored by Hao Jia.

Collection Method

Single-nucleus RNA sequencing (snRNA-seq) of human myocardial tissues.

Time Range

Covers human developmental stages through aging; specific collection dates not provided.

Freshness

Data last updated April 2026, representing recent research.

Geography

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