Lipase Production by Aspergillus Brasiliensis in Solid-State Cultivation of Malt Bagasse

An experimental study evaluated lipase production using Aspergillus brasiliensis 157f in three bioreactor configurations: static flat-bed, plugged-flow bed with forced water-saturated aeration, and a pilot-scale rotating drum bioreactor. The research optimized parameters to a pH of 7.7, 11.3% soybean oil addition, and a culture temperature of 32.7°C, achieving a highest enzyme activity of 9.8 U.g⁻¹ substrate. The fermented medium was lyophilized into a solid enzymatic preparation (SEP) with 7.35 U.g⁻¹ activity for testing in esterification and transesterification reactions.

Use Cases

• Optimizing bioreactor design for enzyme production based on reported activity levels across three configurations.
• Modeling the effect of pH, oil content, and temperature on lipase yield using the provided optimal parameters.
• Developing cost-effective biocatalysts for esterification reactions based on the solid enzymatic preparation (SEP) methodology.
• Comparing solid-state cultivation performance using different aeration and agitation strategies described in the study.

Strengths

• Reports specific optimal conditions: pH 7.7, 11.3% soybean oil, and 32.7°C culture temperature.
• Quantifies enzyme activity with concrete measurements: 9.8 U.g⁻¹ substrate in the best configuration and 7.35 U.g⁻¹ for the solid enzymatic preparation.
• Compares results across three distinct bioreactor configurations: static flat-bed, plugged-flow bed, and pilot-scale rotating drum.

Limitations

• Row count and dataset scale are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Paulo Eichler via paperswithcode

Collection Method

Experimental data from solid-state cultivation studies in laboratory bioreactors.