LT-ZHILIC: 471 Metabolite Standards and Human Plasma Metabolomics Data

471 metabolite library standards were separated using a low-temperature HILIC method for LC-MS metabolomics. The dataset, authored by Yifan Liu and last updated in May 2026, contains results from applying this method to human plasma and cell extracts. It demonstrates stability over 100 consecutive injections and was used to characterize metabolic consequences of nutrient deficiencies.

Use Cases

• Benchmarking chromatographic separation performance based on the 471 metabolite standards library.
• Analyzing metabolic perturbations in cell culture based on the application to glutamine and pyruvate deficiency samples.
• Evaluating retention time stability for LC-MS workflows based on the reported stability over 100 injections.
• Comparing column performance for polar metabolite analysis based on the evaluation of Z-HILIC, amide, and ZIC-pHILIC columns.

Strengths

• Includes data for 471 metabolite library standards, providing a reference for identification.
• Demonstrates method stability over 100 consecutive injections, as stated in the description.
• Compares performance across different column chemistries (Z-HILIC, amide, ZIC-pHILIC).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 37.7 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Data generated from LC-MS analysis of metabolite standards, human plasma, and cell extracts using an optimized HILIC method.

Freshness

Last updated 2026-05-28 05:29:29