MALDI Imaging Mass Spectrometry of a Healthy Mouse Kidney at 150 µm Resolution

MALDI imaging mass spectrometry data from a healthy C57BL/6 mouse kidney tissue section. The dataset was generated using an Autoflex III Smartbeam instrument in negative ion linear mode, with a spatial resolution of 150 micrometers. It was prepared by Adrien Nyakas using a specific tissue washing and matrix application protocol.

Use Cases

• Spatial mapping of molecular distributions based on the 150 µm resolution imaging data.
• Method development for tissue preparation and analysis based on the detailed sublimation and recrystallization protocol.
• Analysis of lipid or metabolite profiles in kidney tissue based on the negative ion mode mass spectrometry.
• Benchmarking computational tools for processing MALDI imaging data from a defined, healthy tissue sample.

Strengths

• Specific spatial resolution of 150 micrometers is documented.
• Detailed instrument and sample preparation protocol is provided, including matrix type and tissue washing method.
• Mass range (m/z 2000 - 20000) and ion mode (negative) are explicitly stated.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and file size are unknown, which may limit suitability assessment.

Provenance

Source

Adrien Nyakas via paperswithcode.

Collection Method

Data was generated using an Autoflex III Smartbeam MALDI-TOF instrument on a snap-frozen mouse kidney tissue section.