CyMeta-ImaGating: Single-Cell Metabolic Atlases for Immune and Cancer Cells

CyMeta-ImaGating is a dataset of single-cell metabolic atlases generated using metabolomic cytometry with an image-enabled gating strategy. The data includes verified single-cell profiles from scraped HeLa cell suspensions, spleen, liver, and cancer cells, demonstrating transitions in metabolic states after pathogen activation and drug treatment. The dataset, authored by Yuanyi He and last updated in March 2026, is shared under a CC-BY-NC-4.0 license.

Use Cases

• Classifying major immune cell types (B, T, NK cells) based on metabolic profiles from spleen cell atlases.
• Analyzing transitions of metabolic states in immune cells after pathogen activation.
• Characterizing drug-specific metabolic effects, such as arginine upregulation during apoptosis, using liver cell atlases.
• Investigating drug-responsive metabolic states in cancer cells that remain robust despite reduced single-cell rates.

Strengths

• The image-enabled gating strategy reportedly increased the proportion of verified single-cell profiles from 28% to 91–100% in scraped HeLa cell suspensions.
• Data demonstrates the method's application across multiple biological contexts: spleen, liver, and cancer cells.
• The dataset is structured in CSV format, a widely accessible tabular format.

Limitations

• Row count and column-level documentation are unknown, which limits suitability assessment and requires manual inspection after download.
• The dataset is small in scale at 161.6 KB, indicating limited scope.
• Data may reflect bias inherent to the specific experimental samples and conditions described.

Provenance

Source

figshare, authored by Yuanyi He.

Collection Method

Generated using CyMeta-ImaGating, a metabolomic cytometry technique with image-enabled gating matching metabolomic profiles to brightfield single-cell images.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-03-24 03:29:27; freshness should be verified.

Geography

Spatial coverage not specified.