hSOX10 Proximal Protein Interactome in Human Melanoma Cells

A 2026 study by Catherine M. Newsom-Stewart provides a high-confidence mapping of proteins interacting with the transcription factor SOX10 in melanoma. The dataset includes 847 candidate interactors identified via proximity-dependent biotinylation and mass spectrometry, refined to 180 high-confidence and 124 biologically relevant candidates. This resource is intended to generate testable hypotheses about SOX10 regulatory networks and therapeutic vulnerabilities in melanoma.

Use Cases

• Prioritizing candidate proteins for functional validation based on the evidence-based prioritization framework described.
• Analyzing functional enrichment of transcriptional regulators and chromatin-modifying complexes associated with SOX10.
• Investigating transcriptomic coexpression patterns between SOX10 and its candidate interactors in melanoma.
• Studying genomic co-occurrence of candidate interactor genes with SOX10 in melanoma samples.

Strengths

• Identifies 847 melanoma-enriched candidate SOX10 interactors from an unbiased capture method.
• Refines the list to 180 high-confidence candidates using stringent statistical filtering and contaminant profiling.
• Further prioritizes 124 biologically relevant candidates by integrating orthogonal genomic, transcriptomic, and functional data.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is derived from a single melanoma cell line (A375), which may limit generalizability to other melanoma contexts.

Provenance

Source

Catherine M. Newsom-Stewart via figshare

Collection Method

Proximity-dependent biotinylation (miniTurbo) coupled with mass spectrometry in A375 melanoma cells.

Freshness

Last updated 2026-05-12 09:17:08; freshness should be verified.