hSOX10 Proximal Protein Interactome in Human Melanoma Cells

Catherine M. Newsom-Stewart mapped the proximal protein interactome of the transcription factor hSOX10 in A375 melanoma cells using miniTurbo proximity-dependent biotinylation and mass spectrometry. The dataset contains 847 melanoma-enriched candidate interactors, refined to 180 high-confidence and 124 biologically relevant candidates. This resource was last updated on 2026-05-12.

Use Cases

• Prioritize candidate proteins for functional validation in melanoma based on the 124 biologically relevant interactors.
• Study transcriptional regulation and chromatin-modifying complexes based on the functional enrichment of identified proteins.
• Investigate therapeutic vulnerabilities in melanoma by analyzing the hSOX10-associated regulatory network.
• Benchmark protein-protein interaction discovery methods using the high-confidence list of 180 candidates.

Strengths

• Identifies 847 melanoma-enriched candidate hSOX10 interactors from an unbiased capture method.
• Provides a refined list of 180 high-confidence candidates using stringent statistical filtering and contaminant profiling.
• Further refines the dataset to 124 biologically relevant candidates integrated with orthogonal transcriptomic, genomic, and chromatin context.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is derived from a single cell line (A375), which may limit generalizability to other melanoma contexts.

Provenance

Source

Catherine M. Newsom-Stewart via figshare.

Collection Method

miniTurbo proximity-dependent biotinylation coupled with mass spectrometry in A375 melanoma cells.

Freshness

Last updated 2026-05-12 09:17:09; freshness should be verified.