MaRNAV_Ethiopia_Code_Script: Matryoshka RNA Virus Sequences from Plasmodium Vivax

Ethiopian Plasmodium vivax samples (Pv01–Pv03) were analyzed to derive consensus sequences for Matryoshka RNA virus (MaRNAV) segments. The dataset includes nucleotide and protein sequences, BLAST validation reports, phylogenetic alignments, and maximum-likelihood tree outputs. It was created by Frank Nyondo and last updated on 2026-04-19.

Use Cases

• Phylogenetic analysis of MaRNAV sequences based on provided protein alignments and Newick tree files.
• Validation of sequence identity using the included raw BLASTX and BLASTP reports.
• Comparative genomics of viral segments based on the consensus nucleotide and translated protein ORFs.
• Reproducing downstream analyses using the provided Python (PY), shell (SH), and R scripts.

Strengths

• Includes raw BLAST reports and a compact TSV top-hit summary for transparency.
• Provides manuscript-ready phylogram figures highlighting Ethiopian sequences and their closest relatives.
• All outputs are derived from publicly available data (SRR17069092, SRR17069099, SRR17069100).
• Released under a permissive CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (56.7 KB), indicating a limited scope of analysis outputs.

Provenance

Source

Secondary analysis of publicly available paired-end RNA-seq runs from Plasmodium vivax samples in Ethiopia.

Collection Method

Reads were quality-trimmed, mapped to MaRNAV reference segments, and variant/consensus calling was performed.

Freshness

Last updated 2026-04-19 08:23:00; freshness should be verified.

Geography

Ethiopia